Evaluation methods for small vascular network distribution and counting around the knee joint in rats

doi:10.3969/j.issn.2095-4344.2014.09.002

Abstract

Abstract:

BACKGROUND: With the deepening of bone tissue engineering research and bone metabolism understanding, it is a hotspot to analyze the blood supply and nutritional status of tissue-engineered bone.
OBJECTIVE: To compare different methods for evaluating small vascular network distribution around the knee joint in rats in order to provide a guideline for the study of microvascular network in tissue-engineered bone.
METHODS: Eighteen Sprague-Dawley rats were randomly divided into three groups, with six rats in each group. Three commonly methods were used to evaluate the small vascular network around the knee joint in rats: immunohistochemistry analysis, angiography analysis, and CT scans and reconstruction analysis.
RESULTS AND CONCLUSION: The microstructure of vascular network could be observed by immunohistochemistry, but the spatial distribution of vessels could not be evaluated. The spatial distribution of vessels could be showed by angiography and CT scans. However, some of micro vessels were showed unclearly by CT scans. The number of blood vessels detected by immunohistochemistry was (26.50±3.02) vessels, significantly higher than those detected by angiography and CT scans that were (14.12±1.47) and (9.00±1.79) vessels, respectively. Combination of immunohistochemistry and angiography can evaluate the microvascular network at microscopic and macroscopic levels, which can provide the whole information of the vascular network.

中国组织工程研究杂志出版内容重点：人工关节；骨植入物；脊柱；骨折；内固定；数字化骨科；组织工程

全文链接：

Key words: knee joint, arterioles, microvessels, angiography

CLC Number:

R318

Wang Le, Lou Ai-ju, Ding Qiang, Yang Bo, Chen Tao, Tang Long, Zhang Bo, Yin Biao, Song Ting, Zhang Zhi. Evaluation methods for small vascular network distribution and counting around the knee joint in rats[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(9): 1319-1324.

Figures/Tables 2

2.1 SD大鼠膝关节周围微小动脉网的分布情况免疫组化染色后可见膝关节周围有较丰富的微小血管分布，主要营养周围肌肉组织，小动脉形态清晰(图1A)，部分微动脉与微静脉的辨认较困难。血管造影摄片后可以显示SD大鼠膝关节周围血管网的分布整体情况，可见膝关节周围血管网主要起于股动脉，在膝关节上方约股骨髁水平的内后方分为数支小动脉，营养膝关节周围的肌肉组织(图1B)，并有一二支细小分支进入股骨远端或胫骨平台，营养该处骨组织，但该分支在不同大鼠间存在变异。在摄片组中，显影检测出该分支的大鼠有3只，占50%。膝关节周围血管网中最大的一支血管在小腿后方的肌肉组织中延续下行，移行为胫后动脉，主要营养下肢的肌肉和骨组织。 2.2 SD大鼠膝关节周围微血管计数在免疫组化检测组中，膝关节横断面切片中可见褐色深染的部位，考虑为微血管所在的位置(图1A)，在×400镜下可见管状样微血管结构，根据软件计算，膝关节周围微血管网分支为(26.50±3.02)条，其中血管直径为(300.00±119.83) μm，血管形态不一，软件计算时对于微动脉与微静脉的辨认则比较困难。在血管造影摄片组中，膝关节周围可见数支微血管显影，其中长度超过1mm并可计数的血管数为(14.12± 1.47)条，计数的血管直径平均为(608.33±274.62) μm (图1B)。微血管网自股动脉分出后成爪牙状分布于膝关节周围软组织中，有一两支细小分支营养股骨远端和胫骨平台，最大分支向远端续于胫后动脉。在CT扫描重建组中，在膝关节后方可见膝部的动脉发出数支细小分支，环绕膝关节周围，并营养周围肌肉组织和骨组织，可计数的微血管数为(9.00±1.79)条，平均直径为(905.00±174.56) μm，部分微血管显影不完整或不清晰，计数相对困难(图1C)。"

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